ZFIN Image: Wang et al., 2022, Figure 3<tag0:x xml:space='preserve'>. </tag0:x>

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Figure 3.

Tg(drl:hoxa9;hsp70:meis1) inhibits myeloid, erythroid and lymphoid differentiation and the formation of HSCs and myeloid progenitors. (a) Tg(drl:hoxa9;hsp70:meis1) was heat shocked at 24 hpf, and ubiquitous GFP expression was induced after HS. GFP was also expressed in the eyes under the control of the ∂-crystallin promoter. (b) Tg(drl:hoxa9;hsp70:meis1) embryos and control siblings were heat shocked at 24 hpf and assayed by WISH for different genes in zebrafish haematopoiesis at different stages, runx1 (2 dpf), c-myb (3 and 5 dpf), mpx (3 and 5 dpf) and mfap4 (3 and 5 dpf). (c) Neutral red staining of macrophages in embryos at 3–5 dpf. (d) Sudan black staining of neutrophils in embryos at 3–5 dpf. (e) WISH assay for rag1 at 5 dpf. (f) WISH assay for erythroid gene, gata1 in embryos at 2 dpf. (g) Benzidine staining of haemoglobin in mature erythrocytes in embryos at 5 dpf. (h) The quantification of WISH results in (b) (n = 50–100 embryos per group). Data are represented as mean ± s.d. *p < .05, **p < .01. NS means no statistical significance. HS, heat-shock; dpf, days post-fertilization; WISH, whole-mount in situ hybridization.

Acknowledgments

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