ZFIN Image: Wang et al., 2022, Figure 1<tag0:x xml:space='preserve'>. </tag0:x>

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Figure Caption

Figure 1.

Transgenic zebrafish express hoxa9 under the control of drl regulatory element. (a) The schematic representation of Tg(drl:hoxa9) expression with ∂-crystallin::Venus GFP reporter gene. A GFP variant is expressed in the eyes under the control of the ∂-crystallin lens promoter (white arrowheads). Whole-mount immunofluorescence staining with anti-myc antibody indicated the overexpression of hoxa9 in blood cells (white arrows). (b) WISH assay for HSC gene, runx1 in embryos at 36 hpf-3 dpf. (c) WISH for myeloid progenitor gene, c-myb in embryos at 36 hpf-5 dpf. (d) WISH for neutrophil gene, mpx in embryos at 3–5 dpf. (e) WISH for macrophage gene, mfap4 in embryos 3–5 dpf. (f) WISH for erythroid gene, hbbe1 in embryos 3 dpf. (g) Quantification of WISH results in (b–f) at 36 hpf-5 dpf (n = 50–200 embryos per group). Data are represented as mean ± s.d. *p < .05, **p < .01, ***p < .001. drl, draculin; FLM, fluorescence microscopy; WISH, whole-mount in situ hybridization; hpf, hours post-fertilization; dpf, days post-fertilization.

Acknowledgments

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