Figure 6.

Figure 6.

(A) Venn diagrams representing the number of mitochondrial genes from the merged MitoCarta 2.0 (Calvo et al, 2016) and IMPI repository, as well as the zebrafish orthologues of human 483 genes encoding proteins that localize to ER according to the Human Protein Atlas in each chchd4a^−/− set. (B, C) Violin plot showing the distribution of log₂chchd4a^−/− gene enrichments for genes grouped by their location within mitochondria, including also ER genes; (C) gene enrichment analysis for MIA substrates in transcriptomic data for WT (blue) and chchd4a^−/− (red) 5 days post fertilization samples. The purple dashed line represents log₂FC = −1 and the green dashed line represents log₂FC = 1. (D) Two sequence logo illustrating motifs detected in 3′UTR regions of MB fraction-enriched transcripts encoding mitochondrial proteins in chchd4a^−/− samples identified by STREME software (Bailey, 2021). (E) Venn diagrams depicting the overlap between the genes identified in WT and chchd4a^−/− MB and HS fractions, respectively. (F) The sequence logo illustrating TISU element found in the 5′UTR regions of transcripts exclusively enriched in the HS upon chchd4a mutation. (G) Distribution of 5′UTR lengths in which TISU element was detected.