PUBLICATION
Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish
- Authors
- Uszczynska-Ratajczak, B., Sugunan, S., Kwiatkowska, M., Migdal, M., Carbonell-Sala, S., Sokol, A., Winata, C.L., Chacinska, A.
- ID
- ZDB-PUB-221026-14
- Date
- 2022
- Source
- Life science alliance 6(1): (Journal)
- Registered Authors
- Chacinska, Agnieszka, Migdal, Maciej, Winata, Cecilia Lanny
- Keywords
- none
- Datasets
- GEO:GSE167587
- MeSH Terms
-
- Animals
- Genes, Mitochondrial*
- Mitochondria/genetics
- Mitochondria/metabolism
- Mitochondrial Proteins/genetics
- Mitochondrial Proteins/metabolism
- Nuclear Proteins/genetics
- RNA, Messenger/genetics
- Saccharomyces cerevisiae/genetics
- Saccharomyces cerevisiae/metabolism
- Zebrafish*/genetics
- PubMed
- 36283702 Full text @ Life Sci Alliance
Citation
Uszczynska-Ratajczak, B., Sugunan, S., Kwiatkowska, M., Migdal, M., Carbonell-Sala, S., Sokol, A., Winata, C.L., Chacinska, A. (2022) Profiling subcellular localization of nuclear-encoded mitochondrial gene products in zebrafish. Life science alliance. 6(1):.
Abstract
Most mitochondrial proteins are encoded by nuclear genes, synthetized in the cytosol and targeted into the organelle. To characterize the spatial organization of mitochondrial gene products in zebrafish (Danio rerio), we sequenced RNA from different cellular fractions. Our results confirmed the presence of nuclear-encoded mRNAs in the mitochondrial fraction, which in unperturbed conditions, are mainly transcripts encoding large proteins with specific properties, like transmembrane domains. To further explore the principles of mitochondrial protein compartmentalization in zebrafish, we quantified the transcriptomic changes for each subcellular fraction triggered by the chchd4a-/- mutation, causing the disorders in the mitochondrial protein import. Our results indicate that the proteostatic stress further restricts the population of transcripts on the mitochondrial surface, allowing only the largest and the most evolutionary conserved proteins to be synthetized there. We also show that many nuclear-encoded mitochondrial transcripts translated by the cytosolic ribosomes stay resistant to the global translation shutdown. Thus, vertebrates, in contrast to yeast, are not likely to use localized translation to facilitate synthesis of mitochondrial proteins under proteostatic stress conditions.
Errata / Notes
This article is corrected by ZDB-PUB-240213-6.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping