ZFIN Image: Hu et al., 2021, Fig. 2

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Fig. 2

Fig. 2. L72 caused dose and time-dependent apoptosis in the G₂/M cell cycle phase in HEL cells. (A) Flow cytometric analysis after propidium iodide staining at 24 and 48 h of incubation, at the indicated doses of L72 treatment. (B) Densitometry of the cells undergoing apoptosis at various stages of the cell cycle at an indicated time interval of L72 incubation. Data were represented as mean � SD (n = 3). (C) HEL cells were treated by L72 at indicated doses and stained with Hochest 33258, depicting the presence of damaged nuclei through a fluorescence microscope (100X). (D) The cell cycle of HEL cells was analyzed in flow cytometry after propidium iodide staining at 24 and 48 h of incubation. (E) Densitometry of the cells undergoing apoptosis at various stages of the cell cycle at an indicated time interval of incubation. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, versus control.

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