ZFIN Image: Liu et al., 2022, Fig. 7

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Fig. 7

TGF-β promotes apoptosis in the eyes of zebrafish embryos during development. A As shown by the white triangles and yellow arrows, the bright green fluorescence from AO staining indicates the apoptosis in eyeballs in each group at 3 dpf and 5 dpf, respectively. The bright-field picture shows the side view of each group of embryos in which the eyeball is magnified in equal proportion. The scale bars refer to 200 μm. B Number of apoptotic cells at 3 dpf per eye from AO staining (n = 26, 54, 54, 45, and 39 from left to right). C Lateral area of the eyeball in each group (n = 11, 20, 21, 20, and 16 from left to right). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. β-Actin was used as the internal control. WT, wild-type. MU, lrpap1 homozygous mutant. dpf, days post-fertilization. 2 m, two months. 3 m, three months

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