ZFIN Image: Zhao et al., 2022, Fig. 7

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Figure Caption

Fig. 7

Bax^tm1Sjk mutant mice exhibit compromised microglial maturation and Notch signaling.

a Immunofluorescence of BAX (red) with SOX2 (gray) and F4/80 (green) in the sagittal sections of E14.5 mice brains. b Quantification of IBA1⁺ and F4/80⁺ cell density in E14.5 WT and Bax^tm1Sjk mice midbrains. Each dot denotes one mouse (n = 5 mice in each group). Three sections per mouse were quantified. c, d 3D reconstructions of confocal z-stacks (c) and measured volume (d) of IBA1⁺ cells in E14.5 WT and Bax^tm1Sjk mice mesencephalon (n = 3 mice in each group). Each dot in (d) denotes one cell. Ten cells per mouse were quantified. e Transcriptional levels of P2ry12, Hexb, C1qa, Slc2a5, and Fcrls in CD11b^hiCD45^lo cells from E14.5 WT and Bax^tm1Sjk mice mesencephalon. The data are from three independent experiments. Each dot represents an independent experiment. f Immunofluorescence of p-CaMKII (left) and p-CREB (right) in brain sagittal sections of E14.5 WT and Bax^tm1Sjk mice. g WB of p-CaMKII, p-CREB and GAPDH in the brain cells from E14.5 WT and Bax^tm1Sjk mice. The experiments were triply repeated. The results were similar. h Immunofluorescence of HuC/HuD (green) and DLL3 (red) in the brain sagittal sections of E14.5 WT and Bax^tm1Sjk mice. i, j WB of DLL3 and β-catenin (i), NICD1 and Hes1 (j) in the brain cells from E14.5 WT and Bax^tm1Sjk mice. The experiments were triply repeated. The results were similar. k Immunofluorescence of F4/80 (green) and Hes1 (red) in the brain sagittal sections of E14.5 WT and Bax^tm1Sjk mice. White arrows indicated the co-stained signals. White arrowheads presented the F4/80⁺ signals only. l The percentage of F4/80⁺ microglia with or without Hes1⁺ signals in (k) (n = 4 mice in each group). Three sections per mouse were quantified. The number in each histogram indicates the average percentage. m A diagram of the Bax-Notch axis in regulating microglial maturation, created with BioRender.com. The small images in the right corners of (a, h, and k) are the enlargement regions (white dotted box). Error bars, mean ± SEM. **P < 0.01; ****P < 0.0001, Unpaired two-tailed Student’s t test. Source data are provided as a Source Data file.

Acknowledgments

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