Activation of Wnt signaling pathway attenuated the adverse effects of Cd2+ on zebrafish early development. (A) Schematic design for the following study. (B,C) High-throughput tracking (representative images) of zebrafish early development, and the duration for embryos in different groups to reach certain stages. (D–F) Heart rate, rate of deformed individuals, and body length of 6 dpf zebrafish in different groups. (G) Representative morphological characteristics of zebrafish larvae in each group. The values are presented in boxplots (median and mean, whiskers show 90% confidence levels) or in histograms (mean ± SEM). The results of either nonparametric test or ANOVA followed by multiple comparison test are listed in Table S2. “*” indicates significant differences (* p < 0.05, *** p < 0.001).
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Full text @ Int. J. Mol. Sci.
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