Figure 1

Figure 1

Testing Apparatus. (A) The open field testing arena consisted of a white plastic cylinder (26.0 cm in diameter) filled to a water depth of 5.0 cm. (B) For the novel object approach test, a multicoloured Lego figurine with a height of 4.25 cm was affixed to the center of the open field arena using a ′1 × 2′ Lego brick. The test arena was partitioned into three zones in EthoVision XT motion tracking software (inner, transition, and outer zones). (C) Diagram of experimental procedure: Individual fish were netted from the housing facility into a dosing beaker for 10-min of terpene exposure, then transferred to the open field arena. After a 10-min trial in the open field test, a Lego figurine was placed into the center of the arena for the 10-min novel object approach test trial.