ZFIN Image: Bhandiwad et al., 2022, Fig. 6.

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Figure Caption

Fig. 6.

vPPN activation correlates with arrest induced by intense stimuli.

(A) Dorsal plane from registered, averaged pERK/tERK ratios in control (n = 12 fish, left) and vibration-exposed fish (n = 12, right). Color shows p/tERK ratio (AU). Bottom: Magnified region around right vPPN. Scale bar, 30 μm. (B) pERK/tERK ratios in vPPN masked regions for control (gray, n = 12) and vibration stimulus-exposed (red, n = 12) conditions. ANOVA F_1,22 = 92.98, P < 0.001. (C) Apparatus used to deliver pulsed water jets at head-fixed zebrafish during calcium imaging. (D) Top: Activity in flow-responsive (n = 41 neurons from 14 fish) and nonresponsive (n = 379 cells) neurons. GCaMP6s fluorescence normalized to coexpressed nuclear-localized red fluorescent protein (RFP; ΔR/R). Vertical lines show flow stimulation period. Bottom: Average ± SEM normalized GCaMP6s fluorescence for responsive (red) and nonresponsive (black) neurons. (E) Location of responsive (red) and nonresponsive (gray) neurons within the vPPN. (F) Change in speed relative to baseline (mm/s) in sibling controls (gray, n = 23 fish) and y334-Gal4, UAS:CoChR-tdTomato (red, n = 22 fish) following 15 s, 60 Hz (50% duty cycle) photostimulation. Effect size is Cohen’s d. ANOVA F_1,45 = 8.196, P = 0.006.

Acknowledgments

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