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FIGURE 5
(A) A schematic diagram for NAC treatment procedures by HR and the effects of NAC with reoxygenation against the hypoxic injury with HR 96 hpf. (B) ROS production/accumulation in zebrafish head at 96 hpf in normoxic control (n = 16), normoxic NAC (n = 17), HR control (n = 14), and HR NAC (n = 14) analyzed by two-way ANOVA followed by Tukey’s post hoc test and presented fold changes compared to normoxic control. (C–E) Different expression of proapoptotic genes (p53, caspase9, caspase3, and bax) by HR, including HR NAC (n = 6/group, triplicated) and HR control (n = 6/group, triplicated), normoxic NAC (n = 6/group, triplicated), and normoxic control (n = 6/group, triplicated) analyzed by two-way ANOVA followed by Tukey’s post hoc test and presented by percent changes. (F) The Z-stack, confocal images of TUNEL+ and the number of (G) anti-TH, (H) TUNEL+, and (I) anti-TH/TUNEL+ at 96 hpf (174.22 μm × 174.22 μm × 30 μm3) in normoxic control (n = 5), normoxic NAC (n = 6), and HR control (n = 5), and HR NAC (n = 6) analyzed by two-way ANOVA followed by Tukey’s post hoc test. *p < 0.05, **p < 0.01, and ***p < 0.001.