FIGURE 4

Parapineal size differs significantly between Kaga and iCab embryos. Dorsal views focused on the diencephalon of four dpf embryos with anterior to the top. (A–D) otx5l and sox1a are expressed in pineal/parapineal of both iCab and Kaga embryos. (A,B,E) The parapineal expression domain of otx5l is significantly larger in Kaga embryos compared to iCab embryos, whereas no size difference of the otx5l expression domain in the pineal alone or in the pineal/parapineal complex together was detected (n_iCab = 5; n_Kaga = 5). Bar graphs show the means of the pineal complex, pineal and parapineal areas. Error bars represent SEM (standard error of the mean).