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Fig. 3.
(A) Schematic of intersectional ablation strategy to parse y318-Gal4 expression. (B) Maximum projections of y318-Gal4 (red) and y520-Cre (cyan) expression patterns. Bottom: Dorsal and sagittal views of neurons labeled by NTR in y318-Gal4/y520-Cre intersect (mean from n = 3 fish). (C) Swim speed change (mm/s) after stimulus in y318-Gal4/y520-Cre–ablated fish (red, n = 12) and sibling controls (gray, n = 9). Effect size is Cohen’s d. t test P = 0.01. (D) Confocal slice of cerebellum showing y318-Gal4;UAS:kaede (green) and NeuroD-eGFP (red), which labels cerebellar granule cells. Scale bar, 50 μm. (E) Confocal slice of cerebellum showing y318-Gal4;UAS:kaede (green) and gad1b:RFP (magenta; GABAergic neurons). Scale bar, 50 μm. (F and G) Confocal slice of y256-Gal4;UAS:eGFP-CAAX–labeled neurons (magenta) from SAG (magenta arrowhead) coregistered with y318-Gal4;UAS:kaede (green). White arrowhead: neurite termination of y256-Gal4 neurons. Inset: imaged area. Scale bar, 30 μm. (H and I) Confocal slice of y397-Gal4;UAS:eGFP-CAAX–labeled neurons from the anterior and posterior lateral line ganglia (red) coregistered with y318-Gal4;UAS:kaede (green). White arrow: y397-Gal4 neuron termination. Scale bar, 30 μm. (J) 3D reconstruction of SAG neurons from (F) and (G) (magenta), aLLG and pLLG neurons (red) from (H) and (I), and y318-Gal4;UAS:kaede (green). Scale bar, 50 μm. (K) Effects of removing visual (dark; n = 17 control, n = 18 dark), auditory (SAG Abl; n = 23 control, n = 24 ablated), lateral line (lateral line abl; n = 27 control, n = 26 ablated), combined SAG and lateral line (n = 18 control, n = 16 ablated), and somatosensory cues (Trigeminal abl; n = 16 control, n = 9 ablated) on arrest. t test, **P < 0.01, ***P < 0.001. Abbreviations: aLLn, anterior lateral line; EG, eminentia granularis; pLLn, posterior lateral line; SAG, statoacoustic ganglion; D, dorsal; R, rostral; M, medial; L, lateral.