Fig. 2

A. Representative cell-attached single-channel recordings of GlyR openings elicited by 100 mM taurine and 100 mM GABA, respectively.

B. Fractions of GlyR particles in the open, closed, desensitized and expanded-open states of the glycine, taurine and GABA complexes of the SMA-solubilized receptor, calculated by RELION and cisTEM as described in the Methods. Error bars represent SEM.

C. Whole cell, dose-response data for glycine, taurine and GABA. EC₅₀ for glycine, taurine and GABA are 190 ± 20 ?M, 1050 ± 80 ?M and 28.4 ± 0.9 mM, respectively. Error bars represent SEM and n?6 cells for all experiments. Curves are normalized to the glycine maximum current recorded in each cell.

D-F. Distances (in angstrom) and conformation of residues ?2?P and 9?L in the taurine-bound, SMA-solubilized receptor in the open, desensitized and closed states.

G-I. Ion permeation pathway for taurine-bound states, calculated using HOLE and plotted as in Figure 1.

J-K. Pore radius as a function of distance along the pore axis for taurine-bound states (J) and GABA-bound states (K) of the SMA-solubilized receptor.

See also Figures S1, S3?S5, Tables S1?S3, S5, S7, Methods S1?S2 and Data S1.