Imaging of epileptic seizure propagation in 5 dpf zebrafish larvae. (A) Optoacoustic tomography (OAT) images at 3, 8, 14, and 50 s after the beginning of the acquisition–maximum intensity projections (MIPs) along the z direction are shown. E, eyes; B, brain; DF, dorsal fin. (B) Corresponding FL images. (C) Temporal profiles of the differential OA [ΔOA/OA0 = (OA–OA0)/OA0, green and blue plots] and the differential FL [ΔF/F0 = (F–F0)/F0, light green and light blue plots] signals for two different points in the hindbrain (green and light green arrows) and forebrain (blue and light blue arrows). Scalebars–2 mm.
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