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Fig. 6
Cases for the biological significances of the sex specific AS events. (A) The differentially spliced transcripts of ncoa5 were validated in adult (6 mpf) zebrafish gonads (O for ovaries and T for testes) by RT-PCR. (B) Multiple protein sequence alignment of RRM1 from Tachysurus fulvidraco (T.f.; XP_027002460.1), Danio rerio (D.r.; XP_005166217.1), Cyprinus carpio (C.c.; XP_018925131.1), Carassius auratus (C.a.; XP_026121940.1), Salmo salar (S.s.; NP_001133374.1), Cynoglossus semilaevis (C.s.; XP_024915677.1), Oryzias latipes (O.l.; XP_023810720.1), Paralichthys olivaceus (P.o.; XP_019952347.1), Oreochromis niloticus (O.n.; XP_019214575.1) and Monopterus albus (M.a.;XP_020442329.1) obtained with Clustal Omega, the skip region is marked by red box (grey bottom). The asterisk (*) indicates the identical amino acid residue. (C) 3D protein structure modeling of ncoa5-S and ncoa5-L, with the RRM domain represented by a blue ellipse and the skip region designated by a red box, both of which contain sheet and helical structures.