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Fig. 5
Identification of testis- and ovary-specific AS genes using multi-tissue RNA-Seq data. (A) Heatmap of PSI value of ovary, liver, gut, kidney, spleen, testis, brain, heart and muscle. (B) Protein-protein interaction network. (C) Differentially expressed splicing transcripts of cenpe and mapre1a were validated by RT-PCR in gonads and other tissues from adult (6 mpf) male and female zebrafish. The DNA fragments were sequenced and shown in Figure S6. ‘Gi’: gill; ‘E’: eye; ‘B’: brain; ‘H’: heart; ‘L’: liver; ‘S’: spleen; ‘K’: kidney; ‘G’: gut; ‘M’: muscle; ‘T’: testis; and ‘O’: ovary.
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