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Fig. 4
Sequence characteristics of the AS exons and introns in ovaries and testes. (A) Models of splicing related SNPs in different regions for A3SS, A5SS, ES and RI separately. SNPs were classified by position as four mutually exclusive regions, including splice (3′ss and 5′ss), exon (alternative fragment), gene (the splicing gene) and chromosome (out of the splicing gene). (B) model of different groups of DASGs (ACs:All Candidates, AOCs: All Ovary Candidates, ATCs: All Testis Candidates, oDASGs: Ovary specific gDASGs, tDASGs: Testis specific gDASGs). (C,D) MaxEnt score of 5′ss and 3′ss, (E) GC content and (F) length of alternative fragment in splice type A3SS, A5SS, ES and RI, separately.(G) model of frame-preserving or frame-switching and theory rate and actual rate of gDASGs. Exon length determines whether an alternatively spliced single-exon skip is frame-preserving or frame-switching. We define an alternatively spliced exon as frame-preserving if its length is an exact multiple of 3 nt, as its alternative splicing will not alter the protein reading frame of subsequence exons (top). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.