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Figure Caption
Fig. 5 Figure 5. Stain pairings in double in situ hybridization vary widely in efficacy. Lateral and dorsal views of each gene pair with sense probes for comparison following antisense probes. (A)Cabin1 detected with NBT/BCIP (indigo) obscures atoh1b detected with Fast Red (red). (B–E)Atoh1b detected with NBT/BCIP (indigo). (B–D) Fast Red (red), DAB (brown), and Vector Red (yellow) are not sensitive enough to detect Cabin1. (E) Fast Red/BCIP labels Cabin1 without masking atoh1b. Sense probes do not reveal specific staining. Embryos were manually deyolked. Scale bars = 100 μm.
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