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Fig. 2 Figure 2. Comparing permeabilizing agents. Expression at 24 h postfertilization with (A & B) standard 10 μg/ml proteinase K treatment versus (C & D) 80% acetone permeabilization. Embryos treated with proteinase K stained more rapidly. Embryos were deyolked for clarity. Embryos were naturally pigmented. Scale bar = 100 μm.
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