ZFIN Image: Andreassen et al., 2022, Fig. 3

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Figure Caption

Fig. 3

CT_max precedes the brain-wide depolarization in freely swimming zebrafish larvae. (A) Schematic overview of the experimental setup for calcium imaging in freely swimming, 5-d-old Tg(elavl3:GCaMP6s) zebrafish larvae. (B) Top view of setup with color-coded time projection of a heat-ramp fish?s movements in the setup for 3 min (34 to 35 �C). (C) Representative images of brain fluorescence in the same fish as in B, before (Left) and during (Right) the brain-wide depolarization. (D) CT_max and depolarization-onset temperatures measured in heat-ramp fish (black circles; n = 7) (SI Appendix, Table S5). The bars and error bars indicate the mean and SE.

Acknowledgments

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