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Fig. 5
(A). Japanese medaka auts2a expression during embryonic development. (B). Focus on early embryonic development. For both panels bars represent the mean normalized expression calculated using 4 different pools of embryos. The standard deviation is displayed. Primers surrounding exons 2?3 junction target the full-length isoform. Primers surrounding the exons 18?19 junction target both full-length and short C-terminal isoforms. Comparisons were performed independently for each set of primer. Stages sharing the same letter are not significantly different (p < 0.05).
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