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Fig. 3
Hdac6 activity is required for zebrafish pronephros repair after injury. (A) Development was not affected in embryos incubated in 1 µM (upper panel) or 5 µM (lower panel) solution of the Hdac6 inhibitor CAY10603. (B) Only 60% of the embryos treated with 1 µM solution of the Hdac6 inhibitor CAY10603 (10603) repaired the gap after laser-induced injury. The group size is shown in brackets (Fisher’s exact test, two-tailed). (C) The combined Hdac2, Hdac6, and Hdac8 activities are required for the migratory response after injury. Incubation of 48 h old zebrafish in solution containing CAY10603, PCI-34051 and CAY10683 (5 µM, upper panel; 10 µM, lower panel) did not affect embryonic development. (D) Less than half of the embryos incubated in solution containing CAY10603, PCI-34051, and CAY10683 repaired the tubule after injury. The group sizes are shown in brackets below each group. Individual experiments with mean and standard deviation for each group are displayed. p-values were calculated with Fisher’s exact test (two-tailed).