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Fig. 2
Hdac8 and Hdac2 activities are required for pronephros repair after laser-induced injury. (A) Embryos, incubated in 1 µM PCI-34051 (upper panel) or 10 µM PCI-34051 (lower panel) from 48 to 72 hpf developed normally. (B) PCI-34051 affects the pronephros repair in a concentration-dependent manner. Embryos were incubated in DMSO or increasing concentrations of the specific Hdac8 inhibitor PCI-34051 for one hour before ablation and for 24 h post injury (hpi). (C) Treatment of zebrafish embryos from 48 to 72 hpf with 1 µM or 15 µM solution of the Hdac2 inhibitor CAY10683 did not affect development. (D) Treatment of zebrafish larvae with 15 µM solution of CAY10683 (10683) significantly increased the percentage of embryos that did not repair the gap after 24 h. The numbers of examined embryos are displayed in brackets. The PCI-34051 concentrations (micromolar, µM) are shown below each group. The points represent individual experiments. Mean and standard deviation for each group are displayed. Significance was calculated using Fisher’s exact test (two-tailed).