ZFIN Image: Born et al., 2022, Fig. 4

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Figure Caption

Fig. 4

Heat-shock inducible overexpression of eif4ebp3l. (A) Schematic diagram of eif4ebp3l transgene and its cloning strategy used to achieve the heat-shock inducible overexpression of eif4ebp3l. (B) Embryo of the transgenic zebrafish strain Tg(fli1:eGFP/hsp70l:eif4ebp3l-p2A-tdTomato) 42 h after heat-shock. (C,D) Caudal fin and heart of an adult Tg(fli1:eGFP/hsp70l:eif4ebp3l-p2A-tdTomato) zebrafish 44 h and 24 h after heat-shock treatment. (E) Quantification of eif4ebp3l mRNA expression of Tg(fli1:eGFP/hsp70l:eif4ebp3l-p2A-tdTomato) zebrafish embryos and their wild-type siblings after heat-shock treatment by qRT-PCR. Embryos were heat-shocked at 30 hpf. Values are mean ± SEM and ** refers to p < 0.005. n = 300 per group. Scale bar = 500 µm.

Acknowledgments

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