ZFIN Image: Teranikar et al., 2022, Fig. 1

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Figure Caption

Fig. 1

Isolating and segmenting cardiomyocyte nuclei from contracting heart using the DOG (Difference of Gaussian) filter in combination with the watershed algorithm

(A?D) 48 h postfertilization zebrafish ventricular volume was reconstructed using light sheet microscopy, in order to visualize time-dependent motion of myocardial cardiomyocyte nuclei. Raw volume comprised of fused nuclei clusters (yellow highlighted boxes), exacerbated by tissue scattering (B?D) Zoomed in regions demonstrate fused contours of nuclei, adversely affecting individual nuclei analysis (E) Ventricular volume was processed using the difference of Gaussian (DoG) edge detector in conjunction with the watershed algorithm to distinguish individual nuclei from adjacent neighbors.

(F?H) Zoomed in regions show successful separation of nuclei for aiding cell tracking and counting.

(I?J) 2D lateral and axial views illustrate tissue birefringence resulting in merging of nuclei longitudinally (K-L) Segmented lateral and axial views were reconstructed for qualitative assessment of contour separation of overlapping nuclei. (scale bar = 100 microns), a: atrium, v: ventricle.

Acknowledgments

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