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Fig. 8
Effects of 3d treatment on the zebrafish xenotransplantation model (A). Compound 3d did not induce abnormal phenotypes or developmental anomalies in zebrafish embryos after 24, 48 and 72 h of incubation. DMSO-treated embryos were used as control. (B) Representative images of Tg(fli1:EGFP) zebrafish embryos (blood vessels shown in white) transplanted with DiI + HeLa cells (red). The embryos were treated with the indicated concentrations of 3d for 24 h and then the fluorescence intensity was quantified as depicted in panel (C). Data are expressed as mean ± SD (**** p < 0.001). Scale bar, 200 ?m. Effects of 3d treatment on zebrafish embryos. No abnormal phenotypes or developmental defects were seen in comparison to DMSO-treated embryos (as a normal control) after 24, 48 and 72 h. (B) Effects of 3d treatment on the zebrafish xenotransplantation model. Representative images of Tg(fli1:EGFP) zebrafish embryos (blood vessels shown in white) transplanted with DiI + HeLa cells (red). Embryos were treated for 24 h with DMSO (control group), 30 nM 3d or 300 nM 3d. (C) Histograms represent the fluorescence intensity of the tumor xenografts, indicating total HeLa cells present in each embryo after a 24 h treatment with 3d at the indicated concentrations. Data are expressed as mean ± SD (**** p < 0.001). Scale bar, 200 ?m.