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Fig. 2
qRT-PCR analysis for insulin resistance model validation with (a) akt, (b) pepck, (c) zglut3, and (d) claudin-5a genes expression on the effects of high insulin induction for 250 nM at 72 hpf and re-challenging after 24 h with 100 nM insulin at 96 hpf. Beta-actin, a housekeeping gene, was used for normalization. Data represent the mean ± SEM; n = 3 independent replicates with 20 larvae in each replicate. Statistical significance, as analyzed by One-way ANOVA with Tukey multiple comparison groups test, is expressed as (*) p < 0.05, (***) p < 0.001 and (****) p < 0.0001.
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