Fig. 4
a Experimental setup. b Active leukocytes (white arrow) were distributed in the brains of zebrafish with or without peripheral trauma. Scale bar, 40 µm. c Time-lapse light-sheet imaging showed the active leukocytes (red arrow) around the zebrafish brain in CTRL. Scale bar, 20 µm. d Time-lapse light-sheet imaging showed active leukocytes (red arrow) around the brain (1), inside the brain (2), and invading the brain (3) at 4 dpf. Scale bar, 20 µm. e Time-lapse light-sheet imaging showed the active leukocytes (red arrow) around the brain in il1bMut zebrafish. Scale bar, 20 µm. f Time-lapse light-sheet imaging showed the active leukocytes (red arrow) around the brain in il1bMut zebrafish at 4 dpf. Scale bar, 20 µm.