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Fig. 2

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ZDB-IMAGE-220808-63
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Figures for Zeitler et al., 2022
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Fig. 2

Figure 2.High-calorie diet led to glomerulomegaly and decreased podocyte density. A and B: hematoxylin and eosin-stained paraffin kidney sections revealed marked glomerulomegaly in zebrafish fed a high-calorie diet, without evidence of glomerular sclerosis. Kidneys from the high-calorie and high-calorie, high-fat diet-fed groups had significantly larger glomeruli than those in the normal diet-fed group. C: as evaluated by light microscopy, the high-calorie diet-fed group had a higher total cell number in each glomerulus compared with normal diet-fed controls. D: confocal microscopy of kidneys from transgenic fish also showed no difference in total cell density between fish fed a normal diet and fish fed a high-calorie diet. E: in contrast, glomeruli from fish fed a high-calorie diet had lower podocyte density compared with fish fed a normal diet. Scale bars = 25 µm. In B and C, at least 60 glomeruli from 3–4 fish/group were measured on three sections per fish. In D and E, n = 21 glomeruli per group. Data are presented as means ± SD. Statistical tests were as follows: Welch ANOVA with Dunnett’s multiple comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. HC, high-calorie diet; HCHF, high-calorie, high-fat diet; HF, high-fat diet; ND, normal diet.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Renal Physiol.