Fig. 2
ifi30 enriches in endothelial cells (ECs) and expresses in caudal vein plexus (CVP) region (A) Schematic representation of isolation of ECs from Tg(kdrl:EGFP) zebrafish embryos for RNA extraction and RT-PCR (B) Expressions of kdrl, fli1a, dll4, and ifi30 in ECs are examined by RT-PCR. ef1a is used as the housekeeping gene. M indicates the DNA ladder and the corresponding sizes are marked on the left (C–E) Expression of ifi30 in zebrafish embryos from 24 to 48 hpf (C′-E′) Magnifications of the red dotted boxes in the corresponding (C) (D), and (E) panel, respectively. Ifi30 is specially expressed in the CVP region (indicated with red arrowhead).