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Fig. 8

ID
ZDB-IMAGE-220801-250
Source
Figures for Eugenin von Bernhardi et al., 2022
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Figure Caption

Fig. 8 Confocal microscopic analysis of TH- and Otpa-positive cells in the adult zebrafish preoptic region and diencephalon at levels of the periventricular posterior tuberculum, posterior tuberal nucleus, and caudal periventricular hypothalamic zone. Note that a1/a2-e1/e2 are separate enlarged microphotographs of these transverse sections. The entire preoptic complex shows no cellular co-localization of TH and Otpa (a-b) as demonstrated for the posterior parvocellular preoptic (PPp) and suprachiasmatic nucleus (SC; a-a2; white arrows). Only the pear-shaped magnocellular part of the periventricular posterior tuberculum (TPp-m; b-b2/c-c2) shows cellular colocalization of TH and Otpa, but not the parvocellular nucleus of the periventricular posterior tuberculum (TPp-p), as demonstrated in magnifications (b1-c1/b2-c2, white and yellow arrows, respectively). Similarly, in the posterior tuberal nucleus (PTN) many cells show co-localization of TH and Otpa (d1-d2/e1-e2, white versus yellow arrows). Note complete absence of such overlap in the paraventricular organ (PVO; c1-c2) and the entire caudal hypothalamus (Hc; d1/e1), but TH immunostain in liquor-contacting cells of the ventral posterior recess lining of the Hc-b (e1). In both, TPp-m and PTN many–but not all—cells show colocalization of TH and Otpa. Scale bars = 250 µm (a-e), 100 µm (a1-e1), 50 µm (a2-e2). Abbreviations: ATN, anterior tuberal nucleus; Ha, habenula; Hc-a, caudal zone of periventricular hypothalamus in front of posterior recess; Hc-b, caudal zone of periventricular hypothalamus around posterior recess; Hd/Hv, dorsal/ventral zone of periventricular hypothalamus; LH, lateral hypothalamic nucleus; ot, optic tract; PGa/PGm, anterior/medial preglomerular nucleus; PPp, posterior parvocellular preoptic nucleus; PTN, posterior tuberal nucleus; PVO, paraventricular organ; SC, suprachiasmatic nucleus; TeO, optic tectum; TLo, torus longitudinalis; TPp, periventricular nucleus of posterior tuberculum; TPp-m, magnocellular cells of TPp; TPp-p, parvocellular cells of TPp; VM, ventromedial thalamic nucleus

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.