Figure 1

Figure 1

ELS results in exacerbated stress responses in adulthood. (A) Timeline of ELS and behavior experiments and illustrations of the ELS setup and novel tank test. (B) Representative swim trajectories of an individual control and ELS fish revealing that while control fish explored most of the tank, ELS fish swam more at the bottom of the tank. (C) Percentage of time spent in the top zone each minute throughout the 10-min recording. Over time, control animals gradually explored the top zone more frequently than ELS animals. (D) Compared to controls (n = 25), ELS adults (n = 27) spent less time exploring the top (Unpaired t test, p = 0.0003), and more time exploring the bottom (Unpaired t test, p = 0.0038) and middle (Unpaired t test, p = 0.018) zones. (E) Distances travelled were not different between controls (n = 25) and ELS (n = 27) animals (Unpaired t test, p = 0.46). Error bars show ± standard error of the mean. Asterisks denote statistical significance (*p = 0.05, **p = 0.005). ns denotes no significance.