Fig. 4 Laser ablation of neurons in nMLF impaired the swim activity during M-cell-activation-induced defensive motor sequence (A) Rhodamine dextran retrogradely labeled descending neurons in nMLF before (left) and after (right) two-photon laser ablation. (B) In the control group, two spikes generated in an M-cell triggered the defensive motor sequence of initial escape and subsequent swim in wild-type fish. Laser ablation of neurons in nMLF abolished the swim behavior. (C) Average swim burst number is significantly different between the control group and laser ablation group (Student’s t test; n = 6 fish). Circles indicate individual data points. ∗∗∗p < 0.001.
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