Fig. 2
The effect of different PN samples on neutrophil recruitment in the zebrafish tail fin amputation assay. (A) The counting areas for the quantification of neutrophils, and a representative fluorescence microscopy image of amputation-induced migration of neutrophils in zebrafish larvae at 3 dpf. (B) Schematic of the drug treatment in the zebrafish tail fin amputation experiments. (C) The number of neutrophils at the amputation site at 4 h after amputation upon treatment with different PN samples. (D) The relative inhibition rate of neutrophils after treating with different PN samples. Hpf, hours post-fertilization; Veh, vehicle; Beclo, beclomethasone; RPN, raw Panax notoginseng; RPNE, raw Panax notoginseng extract; SPN, steamed Panax notoginseng; SPNE, steamed Panax notoginseng extract. *p < 0.05 and **p < 0.01.