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Figure 2—figure supplement 4. Functional manipulation using HSV1.

(A) Top: Expression of GFP in the cerebellum after injection of HSV1[UAS:GFP] into the cerebellum of Tg[gad1b:Gal4] fish. Bottom: Expression of TeNT-GFP after injection of HSV1[UAS:TeNT-GFP] into the cerebellum of Tg[gad1b:Gal4] fish. (B) Examples of swimming trajectories (15 min) of individual Tg[gad1b:Gal4] fish that received injections of HSV1[UAS:GFP] (left) or HSV1[UAS:TeNT-GFP] into the cerebellum. Note that the fish injected with HSV1[UAS:TeNT-GFP] covered less territory, showed fewer long straight swims, and showed a tendency to stay lower in the water column. (C) Mean swimming speed and 2D space occupancy of Tg[gad1b:Gal4] fish that received injections of HSV1[UAS:GFP] (left) or HSV1[UAS:TeNT-GFP] into the cerebellum. Plot symbols represent data from individual fish; box plots show median and 25th and 75th percentiles, circles and error bars indicate mean and s.d. over individual fish. N: number of fish. p=0.03 for swimming speed, p=0.0012 for space occupancy, Wilcoxon rank sum test.

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