Figure 2—figure supplement 2.

Figure 2—figure supplement 2.

(A) Expression of GFP and mCherry after injection of HSV1[UAS:GFP & UAS:TVA-mCherry] into the cerebellum of Tg[gad1b:Gal4] fish. In this virus, two expression constructs, UAS:GFP and UAS:TVA-mCherry, are packaged into the same virus particles. Expression is observed in Purkinje neurons and in putative Golgi cells. Note high rate of co-expression of GFP and mCherry. ML: molecular layer; PL: Purkinje layer; GL: granular layer. (B) Expression of GFP and mCherry in the Purkinje layer after co-injection of two independent viruses (HSV1[UAS:GFP] and HSV1[UAS:TVA-mCherry]) into the cerebellum of Tg[gad1b:Gal4] fish. Note that the rate of co-expression was high even though GFP and mCherry were delivered by separate viruses. Note also that the overall expression was sparse, implying that co-expression was unlikely to occur by chance. (C) Percentage of GFP and mCherry-expressing neurons among all fluorescent neurons. Filled circles represent data from individual fish, box plot indicates median and the 25th and 75th percentiles, and open circles indicate mean over individual fish. N: number of fish.