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Fig. 4
Sensitivities of DrHCN4 and DrHCN4L to HCN inhibitors. (A?C) Representative current traces sequentially applied with 0?100 µM of ivabradine. The holding potential was ?30 mV and was hyperpolarized to ?120 mV for 2 s. (D) Ivabradine inhibition curves based on currents of (A?C). DrHCN4 (blue; n = 5), DrHCN4L (red; n = 4) and HsHCN4 (black; n = 4). (E?G) Representative current traces sequentially applied with 0 ?M?1 mM of ZD7288. The holding potential was ?30 mV and was hyperpolarized to ?120 mV for 2 s. (H) ZD7288 inhibition curves based on currents of (E?G). DrHCN4 (blue; n = 5), DrHCN4L (red; n = 5) and HsHCN4 (black; n = 5). (I?K) Representative currents sequentially applied with 0 ?M?1 mM of cesium ion. The holding potential was ?30 mV and was hyperpolarized to ?120 mV for 2 s. (L) Cesium ion inhibition curves based on currents of (I?K). DrHCN4 (blue; n = 5), DrHCN4L (red; n = 5) and HsHCN4 (black; n = 5).