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Fig. 4
TNBS significantly reduces the number of mucus-producing goblet cells in AB but not TU. At 6dpf, control or larvae exposed to 70 μg/mL of TNBS (T70) were euthanized and stained with alcian blue to quantify mucus-producing goblet cells in the intestine. (A) representative images of mid intestinal region of larvae showing goblet cells stained in blue (B) Analysis of the number of goblet cells in the intestines show that in physiological conditions, AB presents a higher number of goblet cells compared to TU (CTR TU vs CTR AB; **P ≤ 0.01), however TNBS exposure causes a significant decrease in the number of goblet cells in AB larvae (CTR AB vs T70 AB &&&P ≤ 0.001), whilst in TU, TNBS does not cause alterations in the number of goblet cells. CTR AB vs. T70 TU, ***P ≤ 0.001; CTR TU vs. T70 AB, ***P ≤ 0.001. Data are presented as mean and standard error of mean and differences were calculated by unpaired t-student test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)