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Fig. 5 Figure 5. Conserved role of zebrafish ythdc2 in male fertility (A) Generation of ythdc2 knockout (Δ46) zebrafish. Heterozygous crosses give rise to animals in the expected Mendelian ratios. (B) Crosses of heterozygous and homozygous ythdc2Δ46/Δ46 knockout males or females with wildtype partners. Crosses with homozygous knockout males do not produce any embryos, indicating that they are infertile. See also Figure S5B. (C) Hematoxylin and Eosin staining of adult zebrafish testes showing that the infertile homozygous ythdc2Δ46/Δ46 fish lack sperm. Scale bar in μm is indicated.
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