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Fig. 7
iCodon potential applications. iCodon can be used to uncover gene expression patters from the coding sequence; or to identify cis-regulatory elements. iCodon can be used to design sequences with neutral mRNA stability, these sequences will be more responsive to other regulatory elements (i.e., miR-430). Exogenous genes that are wanted to be expressed in a vertebrate model organism could result toxic for the cell; by designing coding sequences with a decreased expression, the toxicity level can be reduced. Tissue/cell visualization: fluorescent proteins found in another organism can be optimized for expression in vertebrate species. Injected mRNA (zebrafish embryos) or mRNA vaccine design can be codon optimized to increase the mRNA stability and expression. Expression gradient: iCodon has the potential to design a wide variety of coding sequences, which will show different gene expression patterns.