ZFIN Image: Bielak et al., 2022, Fig. 10

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Figure Caption

Fig. 10

Fig. 10. The changes in $\bar{s_{20 w}}$ $\overset{�}{s_{20 w}}$ derived from AUC of 0.2 mg/ml, 0.5 mg/ml and 1.0 mg/ml concentrations of dOtol1 (A) and hOtol1 (B) in the presence of 10 mM EDTA or 10 mM Ca²⁺. The sedimentation profile is shown in Fig. S4. Panel C represents the dependence of the cumulative radius of dOtol1 and hOtol1 on protein concentration and Ca²⁺ presence estimated with DLS. The fluorescence anisotropy values of ATTO-488-labelled dOtol1 and hOtol1 at increasing Ca²⁺ concentrations are shown in Panel D.

Acknowledgments

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Reprinted from International journal of biological macromolecules, 209(Pt A), Bielak, K., Ho?ubowicz, R., Zoglowek, A., ?ak, A., K?dzierski, P., O?yhar, A., Dobryszycki, P., N'-terminal- and Ca²⁺-induced stabilization of high-order oligomers of full-length Danio rerio and Homo sapiens otolin-1, 1032-1047, Copyright (2022) with permission from Elsevier. Full text @ Int. J. Biol. Macromol.