ZFIN Image: Mesika et al., 2022, Fig. 4

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Fig. 4

ngly1 ^(−/−) larvae displayed loss of axon fibers in the PNS compared to ngly1 ^(+/+). (A) Dorsal view and (B) ventral view (C) Brain (D) Spinal cord of 6dpf larvae (WT) identified by in situ hybridization analysis-X4, X20, confocal Nikon eclipse, n = 10. (E) Brain (F) Spinal cord of Tg (HUC:gal4 uas:mem YFP-mito CFP)- X20, confocal Nikon eclipse, n = 5. (G) ngly1 ^(+/+) control and (H) ngly1 ^(−/−) mutant groups at 6dpf. Sc = spinal cord, Pn = Peripheral nerves, white arrow represents the loss of axon fibers in PNS. X4, confocal Nikon eclipse. (I) Quantification of fluorescence by neurons, t-test (n = 8 in each group ***p < 0.001). Scale bars: black- 50 µm, white- 20 µm.

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Acknowledgments

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