ZFIN Image: Wang et al., 2022, Fig. 2

Image

Figure Caption

Fig. 2

The pancreatic cancer cells show distinct interaction with the innate immune cells in zebrafish. (A) Representative confocal images of Tg(coro1a: GFP)-labeled innate immune cells in PANC1, BxPC3, and AsPC1 xenografts at 1 dpi and 4 dpi. (B) Qualification of innate immune cell percentage in PANC1, BxPC3, and AsPC1 cells at 1 dpi and 4 dpi (no. of innate immune cells/no. of tumor cells � 100). Results are shown as means � SEM from nine different individuals (* p < 0.05, ** p < 0.01, *** p < 0.001, ANOVA). (C) Enlarged confocal images of innate immune cells co-localized with PANC1 and AsPC1 xenografts at 1 dpi and 4 dpi. Arrows point to the co-localized cells. (D) Representative confocal images of Tg(mpx: GFP)-labeled neutrophils in PANC1, BxPC3, and AsPC1 xenografts at 1 dpi and 4 dpi. (E) Qualification of neutrophils percentage in PANC1, BxPC3, and AsPC1 cells at 1 dpi and 4 dpi (no. of neutrophils/no. of tumor cells � 100). Results are from nine different individuals. dpi, days post injection. Scale bars in (A,D), 50 �m; in (C), 25 �m.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.