ZFIN Image: Lai et al., 2022, Fig. 3

Image

Figure Caption

Fig. 3

Evaluation of CA protected against neomycin-induced apoptosis and mechanotransduction channel impairment. (A) Fluorescence photograph of hair cells in neuromasts of 7-dpf transgenic zebrafish larvae treated with 12.5 μM of neomycin for 0.5 h only or pre-treated with 5 μM CA for 2 h. Labeling FM4-64 fluorescence dye that passes through hair’s h mechanotransduction channel was market d as a red-color signal. A comparison of the signal intensity between untreated control, neomycin, and neomycin pre-treated with CA for 2 h showed that CA prevented neomycin-induced mechanotransduction channel impairment. Scale bar: 10 μm. (B) Apoptotic hair cells were marked as light-respotsot (middle le) in the anterior region of the lateral line system after TUNEL staining. The location of the oval window (ov) and eye a were marked by the white circle. Comparison of TUNEL-positive signal intensity between untreated control, neomycin, and neomycin pre-treated with CA for 2 h showed that CA prevented neomycin-induced apoptosis of TUNEL-positive cells. Scale bar: 100 μm.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.