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Fig. 4
Joint clustering of adult human and zebrafish ECs and HSCs. (A) UMAP visualization of nine clusters comprised of adult human liver and adult zebrafish liver ECs and HSCs. (B) Bar graph showing the percentage of cells contributed to each cluster from each species. (C) Dot plot showing differential expression of HSC marker genes split by species to show marker conservation. (D) Immunofluorescent staining for COLEC11 and COL1A1 on WT adult zebrafish liver cryosections (imaged at magnification ×63) and LX-2 cells (imaged at magnification ×20). (E) Immunofluorescent staining for GFP under the hand2 promoter and COLEC11 on WT adult zebrafish liver cryosections (imaged at magnification ×63). zhEC/Imm, endothelial cell/immune cell mix
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