Fig. 5

Fig. 5 Fig 5. The valine residue at position 56 of Wzd is required for its interaction with Wzg, and for the recruitment of Wzg to the division septum.

A) Interaction between Wzg and Wzd, WzdV56A and WzdY82F point mutants was tested by BTH. Black circles indicate median values and brackets show the 25% and 75% percentiles of ?-galactosidase activity measured in cell extracts of at least three independent replicates. Positive control (+): E. coli expressing T18 and T25 fragments linked to leucine zipper domains (zip) that can dimerize; Negative control (-): E. coli expressing untagged T18 and T25 fragments. WzdV56A-T18 lost the ability to interact with T25-Wzg. B) Graph shows the ratio of iGFP-Wzg fluorescence measured at the septum versus the peripheral wall in the S. pneumoniae wzd null mutant strain (BCSJF008, n = 107) and in the wzd null mutant strain expressing Wzd or Wzd point mutants from a constitutive promoter (non-mutated Wzd strain BCSJF010, n = 109, mutant WzdV56A strain BCSJF011, n = 106, and mutant WzdY82F strain BCSJF012, n = 128). WzdY82F, but not WzdV56A, can recruit Wzg to the division septum of pneumococcal bacteria. *P???0.05, **P???0.01, ****P???0.0001.