Fig. 4

Fig. 4 Fig 4. Point mutations in Wzd affect its localization. A) Scheme of Wzd protein indicating the localization, in the extracellular loop, of four mutations that were associated with a mucoid colony phenotype [19]. B) Localization of Citrine fluorescent derivatives of Wzd single-residue mutants expressed in a wzd null mutant strain. Strain BCSMH022, expressing Wzd-Citrine was used as control. V56A and Y82F mutations did not interfere with the ability of Wzd to localize at the division septum upon interaction with Wze. Representative phase contrast (top panels, for visualization of bacteria), fluorescence microscopy (middle panels, for detection of the capsule associated with the bacterial cell surface) and overlay (bottom panels) images of each strain are shown. Scale bar, 2 ?m.