Fig. 3

Fig. 3 Fig 3. The transmembrane and DNA-PPF domains of Wzg are required for its self-interaction and interaction with Wzd.

A) Scheme indicating the 6 different regions of Wzg tested for interaction with Wzd by BTH. B) Interactions between Wzg and the Wzg1-6 constructs were analyzed by BTH. Black circles indicate median values and brackets show the 25% and 75% percentiles of ?-galactosidase activity measured in cell extracts of at least three independent replicates. Positive control (+): E. coli expressing T18 and T25 fragments linked to leucine zipper domains that can dimerize; Negative control (-): E. coli expressing untagged T18 and T25 fragments. *P???0.05. Only the Wzg5 and Wzg6 constructs, which include the three transmembrane domains and the DNA-PPF domain, interact with full-length Wzg and with Wzd.