Fig. 2

Fig. 2 Fig 2. Septal localization of Wzg is dependent on the expression of Wzd/Wze.

Graph shows the ratio of iGFP-Wzg fluorescence measured at the septum versus the peripheral wall in the S. pneumoniae wild-type encapsulated strain (BCSJF005, n = 194), in the cps null mutant (BCSJF006, n = 121), in the wze null mutant (BCSJF007, n = 106), in the wzd null mutant (BCSJF008, n = 107) and in the cps and wzd null mutants expressing Wzd from a constitutive promoter (BCSJF009, n = 112, and BCSJF010, n = 109, respectively). Enrichment of Wzg at the septum is only observed when Wzd is expressed and is localized at the division septum. Solid lines indicate median, and dashed lines indicate 25% and 75% percentiles. Representative phase contrast and fluorescence microscopy images of each strain are shown below the graph. ****P???0.0001. Scale bar, 2 ?m.